シナプス小胞に局在するVAMP2とpH感受性GFP誘導体との融合タンパク質 (synapto-pHluorin, SpH)をコードする遺伝子を神経特異的プロモーターであるThy1.2の制御下に発現させたトランスジェニックマウス。脳のシナプス前終末の生理学的解析および形態学的解析に使用する。SpH mRNAの発現：脳全体 (TV-7, TV-10, TV-34, TV-47), 海馬のCA1領域 (TV-42ライン), 発現していない/弱い (TV-46)。Tgマウスは通常の維持繁殖には特に問題ない。 Developed by Yuchio Yanagawa, Gunma University Graduate School of Medicine and Hiromu Yawo, Tohoku University Graduate School of Life Sciences in 2002-2003. Transgene was injected into the introduced into the pronuclei of B6D2F2 fertilized eggs. The Tg mice were crossed to C57BL/6J. 群馬大学大学院医学系研究科・柳川 右千夫先生, 東北大学大学院生命科学研究科・八尾 寛先生 (2002-2003)。D6D2F1受精卵へTgをインジェクションにより作成。C57BL/6Jマウスと交配により維持。 国立大学法人群馬大学 Yuchio YANAGAWA Fluorescent Proteins/lacZ System <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/sep_2006_mm" target="_blank">Mouse of the Month Sep 2006</A> The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Araki, R., Sakagami, H., Yanagawa, Y., Hikima, T., Ishizuka, T., and Yawo, H. Transgenic mouse lines expressing synaptopHluorin in hippocampus and cerebellar cortex. Genesis 42, 53-60 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 1) The Recipient should use the Material for research studies or education only in his laboratory.<br>2) The Material should be used with prudence and appropriate caution, since not all characteristics of the Material are fully known.<br>3) The Material should not be used in humans.<br>4) If the use of this material results in one or more scientific publications, Dr. Hiromu Yawo and myself (Yuchio Yanagawa) should be included as co-authors at least in the first paper.<br>5) The Material should not be redistributed without my permission.<br>6) The Material should not be used for commercial purpose. 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Araki, R., Sakagami, H., Yanagawa, Y., Hikima, T., Ishizuka, T., and Yawo, H. Transgenic mouse lines expressing synaptopHluorin in hippocampus and cerebellar cortex. Genesis 42, 53-60 (2005).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>1) 利用者は、利用者の研究あるいは教育の目的にのみ本件リソースを使用する。<br>2) 本件リソースのすべての性質がわかっているわけではないので、慎重にかつ適切な注意を払って本件リソースを使用する。<br>3) 本件リソースは、診断目的を含めてヒトに使用してはならない。<br>4) 本件リソースを使用した研究成果を科学雑誌に発表する際には、少なくとも最初の論文に八尾寛博士と柳川右千夫を共著者として加える。<br>5)本件リソースは、柳川　右千夫の許可なしでは、他へ譲渡ができない。<br>6) 本件リソースは、商業目的にしてはならない。 Carrier x Noncarrier [C57BL/6JJcl] Carrier x Noncarrier [C57BL/6JJcl] TV-7, Thy1-SpH transgenic mice (TV-7) TV-7, Thy1-SpH transgenic mice (TV-7) B6.Cg-Tg(Thy1-spH)7Yyan/Rbrc B6.Cg-Tg(Thy1-spH)7Yyan/Rbrc 柳川　右千夫 Vesicular exocytosis at presynaptic terminals is fundamental function for neurotransmission and brain function. The lumen of a secretory vesicle is acidic (pH 5.6), whereas it becomes neutral (pH 7.4) instantaneously upon exocytosis. The intravesicular change of pH can be detected by a fluorescence change of a pH-sensitive derivative of green fluorescent protein (pHluorin). SynaptopHluorin (SpH) is a fusion protein of pHluorin and syntaxin (VAMP2) for targeting to secretory vesicles. Thy1-SpH transgenic mouse expresses SpH in brain under the control of neuron-specific Thy1.2 promoter. This strain is useful for studies of presynaptic terminals in various regions of the brain. The spH mRNAs was expressed in whole brains of TV-7, TV-10, and TV-34 transgenic lines, whereas in TV-42, it was expressed in the specific regions of the hippocampus. Tg mice mice show no obvious abnormality. C（3〜6か月） RBRC01482 Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li><li>GFP Transfer License (<A HREF="https://web.brc.riken.jp/ja/method/link/gfp_conclude">Japanese</A> / <A HREF="https://web.brc.riken.jp/en/method/link/gfp_conclude">English</A>)<br>Please fill in the <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_a.doc">Schedule A</A>, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read <A HREF="https://web.brc.riken.jp/en/wp-content/uploads/form/gfp_schedule_b.doc"> Schedule B</A>. </li></ol> Gunma Univ. true murine Thy-1.2 expression cassette (Caroni, P. J. Neurosci. Methods 71, 3-9,1997), mouse vesicular membrane protein Vamp2 cDNA, Jellyfish pH-sensitive mutants GFP cDNA (pHluorin cDNA(Miesenbck, G., De Angelis, D.A., Rothman, J. E. Nature, 394, 192-195, 1998).) <a href='https://brc.riken.jp/mus/pcr01482'>Genotyping protocol -PCR-</a> C (3-6 months)